ABSTRACT
Objective To investigate clinical effects and safety differences of conventional and low doses of sufentanil combined with ropivacaine for analgesia on painless childbirth.Methods 130 lying-in women with painless childbirth were chosen and randomly divided into two groups including control group (65 cases) with sufentanil for 7.5μg and observation group (65 cases) with sufentanil for 5.0p g combined with ropivacaine;the block onset time,the arrival time at the maximum block level,modified Brcmage score,the Apgar score in 5min after childbirth,the VAS score before and after analgesia and adverse reaction incidence of both groups were compared.Results The sense and motion block onset time and the arrival time at the maximum block level of control group were separately (4.33 ± 1.91) min,(7.50 ± 1.70) min,(14.26 ± 1.50) min.The sense and motion block onset time and the arrival time at the maximum block level of observation group were separately (3.68 ± 1.54) min,(5.73 ± 1.21) min,(9.71 ± 1.15)min.The block onset time and the arrival time at the maximum block level of observation group were significant shorter than control group (t =2.78,3.07,3.47,all P ＜ 0.05).The modified Bromage score of observation group were significant lower than control group (t =2.92,P ＜ 0.05).There was no significant difference in the Apgar score in 5min after childbirth between the two groups(t =1.29,P ＞0.05).There was no significant difference in VAS score before and after analgesia between the two groups (t =1.15,1.02,0.95,0.63,0.87,all P ＞ 0.05).The adverse reaction incidence of observation group were significant lower than control group (x2 =9.24,8.66,12.07,10.45,all P ＜ 0.05).Conclusion Compared with conventional doses of sufentanil,low doses of sufentanil combined with ropivacaine for analgesia on painless childbirth can efficiently accelerate the block onset process and reduce the risk of adverse reactions.
ABSTRACT
Objective To explore the effects of over-expression high-mobility-group A2(HMGA2) on proliferation and cell cycle in primary cultured human growth hormone-secreting pituitary tumor cells .Methods The protein expression of HMGA2 in growth hormone-secreting pituitary tumor cells transfected by HMGA2 overexpression plasmid (transfection group) were detected by Western blot .The effects of the HMGA2 overexpression on cell proliferation ,cell cycle were measured by (cell counting kit-8 , CCK-8) assay and flow cytometry respectively .Results HMGA2 protein of transfection group was higher than the blank load transfection group(tranfectied with pcDNA3 .1 plasmid) after 24 h (P<0 .05) .To compare with the blank load transfection group , cells overexpression of HMGA2 could drastically increase the ability of proliferation at 24 ,48 ,72 ,96 h and increase the S phase rati-o of cell cycle and decrease the G1 phase ratio of cell cycle ,the difference was statistically significant (P<0 .05) .Conclusion Over-expression of HMGA2 increases pituitary tumor cells proliferation ability and promote cells G 1 phase progress .